Fig. 4: AD-6 antibodies recognise gB vaccine, recombinant gB and infected cells but not virions. | Nature Communications

Fig. 4: AD-6 antibodies recognise gB vaccine, recombinant gB and infected cells but not virions.

From: The cytomegalovirus gB/MF59 vaccine candidate induces antibodies against an antigenic domain controlling cell-to-cell spread

Fig. 4

a–d An anti-AD-6 polyclonal antibody was assayed by ELISA for binding to AD-6 polypeptide (a), vaccine gB (b), a commercial recombinant gB produced in E. coli (c) or CMV virions (d). Antibody levels are represented in function of absorbance (450 nm) per concentration of primary antibody in μg/mL and is representative of three independent experiments. Each data point represents mean + SEM of antibodies purified from two immunized rabbits. e Flow cytometry analysis of mock-infected and CMV-infected cells with anti-AD-6 pAb or non-specific rabbit IgG 3 days post infection. Gated on single, live cells and HLA class I expression to identify virally infected cells.

Back to article page