Fig. 2: NET1 filament formation in the presence of heparin oligosaccharides. | Nature Communications

Fig. 2: NET1 filament formation in the presence of heparin oligosaccharides.

From: The dynamic nature of netrin-1 and the structural basis for glycosaminoglycan fragment-induced filament formation

Fig. 2

Representative negative stain images of NET1ΔC (a) in comparison to full-length NET1 (b) upon the addition of HO-dp10. Images show the formation of extended, filament-like structures (scale bar 200 nm). 2D class averages of NET1ΔC filaments (c) from ~3500 picked particles and class averages of full-length NET1 (d) from ~1500 particles. These class averages indicate a tubular assembly with approximate outer diameters of 152 Å (NET1ΔC) and 148 Å (full-length NET1), and repeating units projecting from the outside of the tube walls (arrows). Scale bars are 100 Å. e Double-helical model for NET1ΔC filament assembly as revealed in a low-resolution X-ray structure, 7LER. Crystal packing yields a tube-like double helical structure similar to that observed by negative stain. The outer diameter of the coil is 200 Å. f Additional NET1ΔC units (red), also present in the crystal structure, associate with the coil via an extensive and previously observed LE-LE interface of the SOS antiparallel dimer, 7LRF. g These units would generate the projections observed in the 2D class averages.

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