Fig. 2: A single +5 G > C SNP in the 5’ leader sequence of the small noncoding RNA ssrA is responsible for increased SpeA expression in M1UK. | Nature Communications

Fig. 2: A single +5 G > C SNP in the 5’ leader sequence of the small noncoding RNA ssrA is responsible for increased SpeA expression in M1UK.

From: Detection of Streptococcus pyogenes M1UK in Australia and characterization of the mutation driving enhanced expression of superantigen SpeA

Fig. 2

a, c, e Quantitative real-time PCR determining speA mRNA expression levels in 5448 (M1global), HKU488 (M1global), SP1380 (M1UK), SP1448 (M1UK), SP1380rofA* (three rofA SNPs repaired), SP1380ssrA* (single ssrA SNP repaired), SP1448rofA* (three rofA SNPs repaired), SP1448ssrA* (single ssrA SNP repaired) and 5448ssrA* (single ssrA SNP introduced). Data from at least three biological replicates are presented as mean values ± SD (a n = 3, c n = 5, e n = 5). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons post hoc test (a ****p  <  0.0001; c SP1380 vs. SP1380ssrA* *p  =  0.0395, SP1380rofA* vs. SP1380ssrA* **p  =  0.0037, SP1448 vs. SP1448ssrA* ***p  =  0.0003, SP1448rofA* vs. SP1448ssrA* *p  =  0.0403) and Welch’s t test (e 5448ssrA* **p  =  0.0023). b, d, f Western immunoblot detection of bacteriophage-encoded superantigens SpeA, SSA, and SpeC and DNase Spd1 in culture supernatants (n = 1). Source data are provided as a Source Data file.

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