Fig. 1: Initial Cryo-EM sample screening to select the grid for high-resolution data collection.
From: The translating bacterial ribosome at 1.55 Å resolution generated by cryo-EM imaging services
a Cryo-EM micrographs of the ribosome particles applied to holey carbon grids with (plus carbon, green) and without (no carbon, blue) an additional thin layer of continuous carbon (2 nm). One micrograph out of 919 (plus carbon) or 1082 (no carbon) is shown. b Sample screening processing scheme using cryo-EM data collected overnight (o/n) on a ThermoScientific Glacios electron microscope. Colours as in “a”. c Fourier Shell Correlation (FSC) plots for the data collected on holey grids with and without an additional layer of continuous carbon. d Orientation distribution of the particles from data collected on plus and no carbon grids.
