Fig. 8: IOX1 and recombinant mouse CCL28 exerts protective effects in vivo during IRI. | Nature Communications

Fig. 8: IOX1 and recombinant mouse CCL28 exerts protective effects in vivo during IRI.

From: Inhibition of ALKBH5 attenuates I/R-induced renal injury in male mice by promoting Ccl28 m6A modification and increasing Treg recruitment

Fig. 8

a Schematic illustrating experiment groups and treatment in 4 groups. b m6A dot blot assessed m6A mRNA methylation under IOX1 or CCL28 treatment in I/R-induced AKI mouse model. c, d Serum creatinine and BUN concentrations. e, f Tubular injury score and representative H&E staining image in different groups of renal tissues. g, h Representative flow cytometry dot plots and percentage of CD4+Foxp3+ cells among the CD4+ T cells in IRI mice with or without IOX1 and CCL28 antibody treatment. i Infiltration of Ly6G+ neutrophil and F4/80+ macrophages in different groups of renal tissues (f, i: Magnification: ×200, Scale bar: 100 μm). For c–e and i, n = 4, 5, 5, 5 for Sham, IRI, IRI + IOX1, IRI + CCL28 groups respectively. For h, n = 3, 4, 5, 5 for Sham, IRI, IRI + IOX1, IRI + CCL28 groups respectively. Each data point represents one animal, with unpaired two-tailed Student’s t-test performed. Data are shown as means ± SD.

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