Fig. 3: Disclosing the sequence determinants of the BV activity by mutational analysis.
From: Evolution of enzyme functionality in the flavin-containing monooxygenases
a Scheme depicting the reverse mutational strategy in terms of functionalities as landscapes in the sequence space23. b List of sites mutated in each of the constructed variants. The subsequent rounds of mutations are represented in gray scale. c Conversions catalyzed by tAncFMO1–4 mutants and d dissecting 4′×-tAncFMO1–4. Bars represent mean conversion values and dots the values for each experiment (n = 2 independent experiments). Source data are provided in Supplementary Table 4.
