Fig. 5: Oxygenating intermediate formation for tAncFMO1-4.
From: Evolution of enzyme functionality in the flavin-containing monooxygenases
Spectra were timely recorded to monitor the reaction of reduced tAncFMO1–4 with molecular oxygen in a stopped-flow apparatus. Representative ones are shown (n = 3 independent experiments). The upper left inset shows the deconvoluted spectra fitted into a two-step process \(a\mathop{\to }\limits^{{k}_{1}}b\mathop{\to }\limits^{{k}_{2}}c\) with k1 = 3.2 ± 0.11 s−1 and k2 = 0.0065 ± 0.0001 s−1. The upper right inset shows the change in absorbance between species a (E-FADH2) and b (E-FADOO(H)). Source data are provided as a Source Data file.
