Fig. 5: Neu1-expressing macrophages in the mouse visceral adipose tissue are a potential source of NANA. | Nature Communications

Fig. 5: Neu1-expressing macrophages in the mouse visceral adipose tissue are a potential source of NANA.

From: N-acetylneuraminic acid links immune exhaustion and accelerated memory deficit in diet-induced obese Alzheimer’s disease mouse model

Fig. 5

a Cellular landscape of the mouse visceral adipose tissue (VAT) immune cells across all genotype and diet conditions. UMAP embedding of single nuclei profiles (sNuc-Seq), colored after post hoc cell type annotation. Mice from three independent experiments, sample n: WT CD = 10, WT HFD = 6, 5xFAD CD = 9, 5xFAD HFD = 13. b HFD increased the frequency of VAT macrophages (MACs). Changes in frequency of the other VAT immune cell types are shown in Supplementary Fig. 11e. Annotations are as in Supplementary Fig. 11d, e. Sample n: see a. c Dot plots featuring the expression of sialidase Neu genes (color scale) and the percentage of cells expressing them (dot size) in the overall VAT (left) and VAT immune compartment (right). Of the four mammalian Neu genes, only Neu1 and Neu3 transcripts were detected. d HFD increased the frequency of Neu1-expressing macrophages in the VAT. Sample n: see (a). b, d Statistical analyses: two-way ANOVA followed by Fisher’s LSD post hoc test. Box plots represent the minimum and maximum values (whiskers), the first and third quartiles (box boundaries), the median (box internal line), and the mean (cross). Source data are provided as a Source Data file.

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