Fig. 1: Near full-length genome sequencing in single p24-expressing cells. | Nature Communications

Fig. 1: Near full-length genome sequencing in single p24-expressing cells.

From: Phenotypic characterization of single CD4+ T cells harboring genetically intact and inducible HIV genomes

Fig. 1

a Isolated CD4+ T cells were stimulated with PMA/ionomycin for 24 h in the presence of BFA prior to extracellular and intracellular staining using antibodies specific to the cellular and viral proteins listed in the HIV-Flow panel table. Single p24+ cells were index-cell sorted in individual wells. NFL HIV amplification by nested PCR was performed on each single sorted p24+ cells, followed by PacBio next-generation sequencing. Proviruses were analyzed individually for their genetic integrity. Levels of expression of cellular markers on each individual cell was retrieved and analyzed according to the genetic integrity and clonality of the provirus from this cell. b Representative dot plots of an HIV-Flow staining from participant ART5. Each colored dot represents a single infected cell (p24+) that was index-sorted. p24+ cells are overlaid onto p24− cells in grey.

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