Fig. 4: LAKI controls mechanical allodynia in mice by sensitization of nociceptor excitability.

a Bar graph summarizing the paw withdrawal threshold of mice injected either with saline solution or with LAKI (100 µM, 15 µL) in the dark or after 20 s illumination at 365 nm (magenta). n was obtained from seven independent experiments. Statistical significance was determined by a mixed-effects model with repeated measures followed by Holm–Sidak’s post-test (***p < 0.001). b Graph summarizing the average of the paw withdrawal threshold of mice injected with LAKI (100 µM, 15 µL) relative to mice injected with saline solution, in the dark or after 20 s illumination at 365 nm (magenta). n was obtained from seven independent experiments. Statistical significance was determined by a mixed-effects model with repeated measures followed by Holm–Sidak’s post-test (***p < 0.001). c Representative spinal dorsal horn neuron responses evoked by electrical stimulation (4 mA) before and after 20 s illumination at 365 nm (magenta) in mice injected with LAKI (100 µM, 15 µL). d Raster plot and superimposed post-stimulus histogram of dorsal horn neurons before (black) and after illumination at 365 nm (magenta) in mice injected with LAKI (100 µM, 15 µL). Data were represented as mean ± SEM. The numbers of mice are indicated in parentheses on the graph.