Fig. 1: Ssu72 phosphatase is potentially involved in physiological regulation of thermogenic adipose tissues, especially BAT.

a Various mouse tissues including epididymal white adipose tissue (eWAT), inguinal white adipose tissue (iWAT), and brown adipose tissue (BAT) were extracted from 7–8 weeks old C57BL/6 (WT) male mice, and lysates from tissues were immunoblotted with antibodies against several representative phosphatases, which were classified into four phosphatase groups (n  =  5, biologically independent samples). b Relative mRNA expression levels of Ssu72 in eWAT, iWAT, and BAT from 9-week-old WT mice (n = 5 mice per group). Data are presented as mean ± SEM. Statistical comparisons were made using one-way ANOVA. c Relative mRNA expression levels of Ssu72 in BAT of 7 to 8-week-old WT mice after mild cold exposure (15 °C) (n = 5 mice per group). Data are presented as mean ± SEM. Statistical comparisons were made using one-way ANOVA. d Western blots of lysates from BAT of WT mice housed at RT (23 °C) or exposed to acute cold (4 °C) for 4 h (left) (n = 3 mice per group), with quantification of Ssu72 and UCP1 protein levels (right). Graph shows quantification of Ssu72 protein levels normalized to β-actin protein level using ImageJ. Data are presented as mean ± SEM. Statistical comparisons were made using two-tailed Student’s t test. e Animated images of Ssu72^flox/flox (Ssu72 WT), Adiponectin-Cre, and Ssu72^flox/flox; Adiponectin-Cre mice (Ssu72 aKO). f Representative images of BAT from 11-week-old Ssu72 WT and aKO male mice (scale bar, 5 mm; n  =  10 per group, biologically independent samples from 8–11 weeks old mice). g Hematoxylin and eosin (H&E) staining of representative sections of BAT from 6- and 22-weeks-old Ssu72 WT and aKO male mice (n  =  5 per group, biologically independent samples). For (f) and (g), both male and female mice were used, and no sex-specific phenotype was observed. Source data are provided as a Source Data file.