Fig. 3: Conformation-dependent reaction kinetics for NE sensing.
From: Two-photon fluorescence imaging and specifically biosensing of norepinephrine on a 100-ms timescale
a Normalized fluorescence response dynamics (recorded at 480 nm, excited at 720 nm) of 5 μM aqueous solution of BPS2, BPS3, BPS4, and BPS3 + CB[7] (1 equiv.) with addition of 100 nM NE. For the curve BPS3 + CB[7], an additional 1 equiv. of AdOH was added at the time of 1200 s after the addition of NE. Inset: Enlarged view of the partial curve for the response of BPS3 and BPS4 toward NE. b Partial 2D NOESY NMR spectrum of BPS3 (1 mM in D2O), at 298 K. c IGMH isosurface of BPS3 in folded conformation. d The calculated Hirshfeld Charge of the carbonothioate carbon of BPS3 in folded and stretched conformations, respectively. e Schematic of molecular conformational regulations by covalent or supramolecular approaches.
