Fig. 2: Structural characterization of protocells.
From: Creating complex protocells and prototissues using simple DNA building blocks
A Confocal laser scanning microscope (CLSM) images of compartmentalized Cy3-labeled DNA nanotubes (NT) and fibers (F) inside giant unilamellar vesicles (GUVs), 14 mM MgCl2 DNA nanotubes, and 42 or 100 mM MgCl2 DNA fibers inside POPG GUVs, scale bar 5 µm. B Left to right, comparison of GUVs with encapsulated DNA nanotubes and fibers pre-folded with increasing MgCl2 concentration, all scale bars 2 µm. C CLSM-derived cross section profiles of DNA nanotubes and fibers, membrane-lipid channel (gray line) and DNA channel (green line). D CLSM-derived diameter histogram plots of DNA nanotube and fiber protocells, n = 747 and 189, for NT and F, respectively. E Circularity dot plot of DNA nanotube and fiber protocells, n = 914, 747, 356, 221 and 189, left to right, respectively. Line and error bars represent the mean and standard deviation, respectively. F Hypertonic osmolar stability dot plot of the stated protocells remaining as a percentage at +550 mOsmol. Line and error bars represent the mean and range respectively, from 3 independent repeats.
