Fig. 4: Constructing exoskeleton protocells.
From: Creating complex protocells and prototissues using simple DNA building blocks
A Schematic representation. B CLSM images of exoskeleton protocell, nanotube (NT) cytoskeleton (green channel), cholesterol lipid anchors (orange channel) and hairy fiber (F) exoskeleton (magenta channel), scale bar 2 µm. C Dot plot of fiber exoskeleton fluorescence bound to GUVs either without (−) or with (+) cholesterol lipid anchors. Line and error bars represent the mean and standard deviation, respectively. D CLSM-derived digestion profile of cytoskeleton DNA fibers (magenta line) and exoskeleton fibers (green line) from a single protocell after the addition of DNase I. E 1% v/v human serum induced 6CF rupturing profiles comparing vesicles encased in either fibers (pink line), nanotubes (purple line) or unmodified (gray line). Line profiles represent the averages of three independent repeats.
