Fig. 7: PfACS10 prefers fatty acid species of 18 carbon length. | Nature Communications

Fig. 7: PfACS10 prefers fatty acid species of 18 carbon length.

From: Potent acyl-CoA synthetase 10 inhibitors kill Plasmodium falciparum by disrupting triglyceride formation

Fig. 7

a ACS10cKD parasites were washed 24 h prior to enrichment for trophozoites by MACS purification to allow for reduced levels of PfACS10. Under these aTc washout conditions, there was no visible defect in the ACS10cKd line. FAs form infected erythrocytes were extracted and submitted to gas chromatography-flame ionization detection (GC-FID); results were normalized to total FA. Shown are the mean ± SD of three biological replicates for relevant fatty acids. b 3D7 and ACS10M300I_C were enriched for young trophozoites and exposed to 10 mM MMV665924 or DMSO for 10 h, after which FAs were extracted and analyzed by GC-FID. Shown are the means ± SD of four biological replicates for relevant FAs. Test for significance used two-sided unpaired Student’s t test, a: p = 0.006, b: p = 0.03, c: p = 0.02, d: p = 0.01. The complete data for the GC-FID are available in Supplementary Data 7 and 8. aTc anhydrotetracycline, FA fatty acid.

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