Fig. 3: Ubiquitous biomarker analysis in single EV across different cell lines.
From: Multiplexed analysis of EV reveals specific biomarker composition with diagnostic impact
A High magnification images of EV (obtained from the PANC-1 cell line) for 12 biomarkers. Scale bar: 50 µm B Quantitative analysis. Each graph shows the abundance of the indicated biomarker as a percentage of all EV (error bar: mean ± standard deviation). EV were harvested from PANC-1, CAPAN2, ASPC1, and A549 cells (for other cells, see Fig. S12-S13). The most abundant marker was CD9, followed by CD29. There were considerable differences in marker positivity, even for EV from the same origin. For example, in EV from pancreatic cancer cell lines, CD9 ranged from 20 to 50% in vesicles. Calnexin was used as an exclusion marker. Number of EV analyzed: PANC-1: 8500; A549: 8,100; ASPC1:14,000; CAPAN2: 4600.
