Fig. 6: Mapping of thousands of single EV.
From: Multiplexed analysis of EV reveals specific biomarker composition with diagnostic impact
A Each row represents a single EV and each column a single or combined tetraspanin biomarker. PANC-1 EV are shown on the left and CAPAN2 EV on the right (for extended profiling of other cell-line derived EV, see Fig. S15). Top right: magnified view shows detailed data on individual EV. No clustering was applied in these examples to illustrate the heterogeneity of biomarkers across the large number of single EV. B Dimensionality reduction of the complete dataset involving 19,000 single EV from 4 different cell lines (yellow: CAPAN2, orange: ASPC1, purple: PANC-1 and blue: A549; see Fig. S8). Note that full biomarker multiplexing allows clear separation of EVs from different origins. Limited biomarker multiplexing with CD9, CD47, and EGFR, such as done by spectrally resolved 3-color imaging, does not allow clear separation.
