Fig. 2: Single-cell RNA sequencing of paired diagnosis/post-chemotherapy samples reveals an early proliferative response alongside with persisting quiescent progenitor blasts.
From: Longitudinal single-cell profiling of chemotherapy response in acute myeloid leukemia
A UMAP density plot showing distribution of sequenced NPM1mut AML blasts at day 14 early post-chemotherapy (D14). n = 849 cells. B UMAP density plot showing distribution of sequenced NPM1mut AML blasts at day 30 post-chemotherapy (D30). n = 469 cells. C Tile plot of normalized enrichment scores (NES) from gene set enrichment analysis (GSEA) of hallmark terms on differentially expressed genes (DEG) between D14 or D30 and diagnosis (DX) blasts of NPM1mut AML patients within the indicated clusters (cl., see Fig. 1B for unsupervised clustering). Hallmarks (rows) are grouped by semantic similarity. Columns represent single-cluster, single-patient comparisons between the specified timepoints and are grouped by cluster phenotype (P, progenitor blasts; E, erythroid-like; C, cycling; M, myelo/monoblasts) and timepoint (Day 14; Day 30). Non-significant enrichment results are plotted in light grey (Benjamini–Hochberg adjusted p-value > 0.1). D UMAP density plot showing distribution of sequenced del(7) AML blasts at day 14 early post-chemotherapy. n = 8970 cells. E UMAP density plot showing distribution of sequenced del(7) AML blasts at day 30 post-chemotherapy. n = 3930. F Tile plot of normalized enrichment scores (NES) from GSEA of hallmark terms on DEG between D14 or D30 and diagnosis (DX) del(7) AML blasts within the indicated clusters (cl., see Fig. 1E for unsupervised clustering). P, progenitor blasts; L, LMPP-like blasts; E, erythroid-like; C, cycling; M, myelo/monoblasts), timepoint (day 14; day 30). Non-significant enrichment results are plotted in light grey (Benjamini–Hochberg adjusted p-value > 0.1).
