Fig. 6: Upregulation of the BMP pathway and downregulation of the MAPK pathway by inactivation of Tbx1 at E9.5. | Nature Communications

Fig. 6: Upregulation of the BMP pathway and downregulation of the MAPK pathway by inactivation of Tbx1 at E9.5.

From: Single-cell transcriptomics uncovers a non-autonomous Tbx1-dependent genetic program controlling cardiac neural crest cell development

Fig. 6

a Wnt1-Cre;ROSA-EGFP lineage tracing (green) shows mis-localization and a reduced number of NCCs within the pharyngeal region of Tbx1 null embryos (red arrow) (n = 10). DAPI is in blue. NCCs from the region between the two dashed lines were used for scRNA-seq. b RISC UMAP plot of integrated scRNA-seq data from NCCs of control and Tbx1 null embryos. c UMAP plots colored by clusters from control (left) and Tbx1 null embryos (right). d Stack bar graph shows proportions of NCCs in indicated clusters divided by the total number of cells in control or Tbx1 null embryos. A two-sided proportion z-test was used to evaluate cell proportion differences between control and Tbx1 null embryos (cluster C3: p value = 1.14e-56; C4: p value = 9.24e-15; C5: p value = 1.46e-8; C8: p value = 1.23e-5; C9: p value = 4.32e-3; C12: p value: 0.66) (*p value <0.05; ns not significantly different). Additional information on statistical analysis is provided in the Source Data files. e, f Scatter plots show differential gene expression in cluster C8 (e) and in cluster C4 (f) from control and Tbx1 null embryos. Note the upregulation of genes involved in the BMP pathway (red) and the downregulation of genes in the MAPK pathway (purple). g Violin plots showing differential expression in control (purple) versus Tbx1 null (red) for Msx2, Bambi, Spry1, and Myc in clusters C4 and C8. PA pharyngeal arch, OV otic vesicle, OFT outflow tract. Scale bars: 100 μm.

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