Fig. 7: BMP pathway is downregulated and the MAPK pathway is upregulated in pharyngeal NCCs of Tbx1 null embryos at E9.5. | Nature Communications

Fig. 7: BMP pathway is downregulated and the MAPK pathway is upregulated in pharyngeal NCCs of Tbx1 null embryos at E9.5.

From: Single-cell transcriptomics uncovers a non-autonomous Tbx1-dependent genetic program controlling cardiac neural crest cell development

Fig. 7

a UMAP plots show the expression level (purple) of Msx2 and Spry1 in NCCs split by control and Tbx1 null embryos. b Wholemount immunostaining through Wnt1-Cre;ROSA-EGFP control (n = 7) and Tbx1 null embryos (n = 5) for GFP (green) and phospho-ERK (pERK) (red). Digitally generated sections are shown in b. DAPI is in blue. Arrowheads show reduced expression of pERK in NCCs in the pharyngeal region of Tbx1 null embryo. c Msx2, Bambi, and Egfp wholemount RNAscope in situ hybridization with DAPI of control and Tbx1 null embryos. (Msx2 in control: n = 7, Msx2 in Tbx1 null embryos: n = 3, Bambi in control and Tbx1 null embryo: n = 3) The arrowheads indicate ectopic expression of Msx2 and Bambi in Tbx1 null embryos. d Egfp, Msx2, and Bambi RNAscope assay on transverse sections of control and Tbx1 null embryos (n = 3) showing ectopic and dorsal expression of Msx2 and Bambi in migrating NCCs (red arrowheads). Msx2 and Bambi expression is absent in the proximal part of the PA2 and PA3 of control embryos (white arrowheads). e Fluorescent immunostaining for GFP and P-SMAD1/5/9 on transverse sections of control (n = 5) and Tbx1 null (n = 4) embryos shows increase in P-SMAD1/5/9 in NCCs of PA2-3 (arrowheads). f Schematic representation of control (left) and Tbx1 null (right) sections at E9.5 showing reduced CNCCs contributing to the shortened OFT and ectopic posterior BMP signaling. PA pharyngeal arch, OFT outflow tract, NT neural tube, end endoderm, LV left ventricle. Scale bars: 100 μm.

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