Fig. 3: Screening of nano-lanterns with different binding sites.

a Agarose gel electrophoresis of stability of naked mRNA and different forms of mRNA nano-lantern treated with 10% fetal bovine serum (FBS) for 2, 4, and 12 h, respectively. b Intensity statistical of panel (a), n = 3 independent experiments, data are presented as the mean ± SEM. c Schematic illustration of cell-free translation of mRNA. (d) Western blot analysis of protein expression level of individual mRNA and different forms of mRNA nano-lantern (3, 5, and 7 bs) in the cell-free translation system, n = 3 independent experiments. e Schematic illustration of the construction of FITC labeled mRNA. (f) Intake profiles of FITC labeled nano-lantern (with 3, 5, and 7 bs) in SW480 cell. The gating strategy was provided in the Supplementary Fig. 16a. g Representative western blot analysis of protein expression level in SW480 and SW620 cells treated with individual mRNA and different forms of mRNA nano-lantern (3, 5, and 7 bs) from 3 independent experiments. h Intensity statistical of panel (g), n = 3 independent biological samples, data are presented as the mean ± SEM. i Schematic illustration of screening of nano-lanterns with different binding sites. Panel (c, e, i) created with BioRender.com. Source data are provided as a Source Data file.