Fig. 4: Cellular uptake of Smad4 mRNA nano-lantern. | Nature Communications

Fig. 4: Cellular uptake of Smad4 mRNA nano-lantern.

From: Lantern-shaped flexible RNA origami for Smad4 mRNA delivery and growth suppression of colorectal cancer

Fig. 4

a Flow cytometry analysis of the cellular dynamic uptake of mRNA nano-lantern in SW480 cells at different time points, n = 3 independent biological samples, data are presented as the mean ± SEM. The gating strategy was provided in the Supplementary Fig. 16b. b Representative confocal images of SW480 cells incubated with mRNA nano-lantern at different time points, the images at each time point are from 3 independent experiments. Fluorophore Cy5 was exploited to label the RGD-CS RNA and the FITC was exploited to label the mRNA scaffold. The spearman correlation coefficient (r) is calculated by Image J software to evaluate the association of Lysotraker and mRNA. c Representative confocal images of SW480 cells incubated with individual Smad4 mRNA, lipo + mRNA, and nano-lantern, respectively, from 3 independent experiments. Scale bar: 20 μm. Panel (c) created with BioRender.com. Source data are provided as a Source Data file.

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