Fig. 5: Target ability and intracellular Smad4 expression of nano-lantern in vitro. | Nature Communications

Fig. 5: Target ability and intracellular Smad4 expression of nano-lantern in vitro.

From: Lantern-shaped flexible RNA origami for Smad4 mRNA delivery and growth suppression of colorectal cancer

Fig. 5

a Schematic illustration of targeting experiments by flow cytometry analysis. Created with BioRender.com. b Characterization of the targeting ability of Smad4 mRNA nano-lantern with or without (W.O.) RGD modification by flow cytometry analysis. The gating strategy was provided in the Supplementary Fig. 16c. c Representative western blot analysis of Smad4 protein expression in SW480 and SW620 cells treated with mRNA nano-lantern for different incubation times from 3 independent experiments. d Representative western blot analysis of Smad4 protein expression in SW480 and SW620 cells treated with individual Smad4 mRNA, Lipo + mRNA, and nano-lantern from 3 independent experiments. e Representative immunofluorescence staining of Smad4 in SW480 cells treated with individual Smad4 mRNA, Lipo + mRNA, and nano-lantern from 3 independent experiments. Scale bar: 20 μm. Source data are provided as a Source Data file.

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