Fig. 7: Smad4 transcriptional suppressed the expression of MYC in CRC cells.

a The Smad4 potential binding site in JASPAR software. b Potential recognition sites of Smad4 in the MYC promoter region. c Western blot analysis of Smad4 expression in SW480 and SW620 cells before and after treatment with nano-lantern. d ChIP assays with anti-Smad4 or negative control (anti-IgG) antibodies showed Smad4 binding to the recognition site (n = 3 independent biological samples). e Representative agarose electrophoresis for ChIP analysis of Smad4 binding to the MYC promoter from 3 independent experiments. f Representative western blot analysis of MYC expression in SW480 and SW620 cells after treatment with mRNA, and nano-lantern from 3 independent experiments. g Representative western blot analysis of VEGFC and CXCL5 expression in SW480 and SW620 cells after treatment with mRNA, and nano-lantern from 3 independent experiments. Data are presented as the mean ± SEM. Statistical differences were assessed using one-way ANOVA with Bonferroni multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.