Fig. 4: Structural characterization of the pore-occluded state in lipid nanodiscs.
From: Cryo-EM structures of human Cx36/GJD2 neuronal gap junction channel
a–c Top, cross-sectioned side, and bottom views of the cryo-EM reconstruction map with C1 symmetry imposition. Ribbon representations of the hetero-junctional Cx36Nano-WT (a), Cx36Nano-ΔN8 (b), and Cx36Nano-BRIL-ΔN16 (c) GJCs are also shown. C.L. and E.L. denote cytoplasmic layer and extracellular layer, respectively. The lipid nanodiscs and pore-occluding lipids are displayed as white densities. Cx36 proteins in the FN and PLN states are colored green and yellow, respectively. The NTHs and the π-helix spanning residues 30–33 are colored magenta and black, respectively. Red dotted circles indicate possible locations of flexible NTHs.
