Fig. 4: Antibody responses induced after LASV challenge.
From: Rapid protection induced by a single-shot Lassa vaccine in male cynomolgus monkeys
Detection of A LASV-specific IgM, B LASV-specific IgG, C NP-specific IgG, D GP-specific IgG, and E Measles-specific IgG in animals after the LASV infection by ELISA. Each point represents the mean ± SEM of three samples, except for the CT animals on day 12 (2 samples) and day 14 (one sample). For statistical purposes, the sample of animal CT_2 collected at the time of death on day 11 is represented as being on day 12, and the sample of animal H + 1_1 collected at the time of death on day 13 is represented as being on day 15. A Kruskal–Wallis test was used after testing for normality with a Shapiro-Wilk normality test. F Measles virus neutralizing antibody titers, expressed as reciprocal of the highest dilution blocking infection of Vero NK cells by 250 plaque-forming units (pfu) of MeV-GFP. The bars represent the mean of n = 3 independent samples. G Percentage of seroneutralization measured by plaque reduction neutralization assay with 1/40 dilutions of plasma collected at various time points from D-16, D-8, H + 1, and CT animals (right panel). Samples reaching 50% or more neutralization were considered to be seroneutralizing. The pink square at day 12 in the D-16 graph indicates the percentage of seroneutralization with a 1/200 dilution. Source data are provided as a Source Data file.
