Fig. 1: iDISCO-cleared and 3D-reconstructed images of the median eminence (ME) from M5-GFP mice immunolabeled for GFP (green, n = 3), the vascular endothelial marker CD31 (red), the endothelial permeability marker PV-1 (red), or the tanycyte marker vimentin (magenta).
From: Bitter taste cells in the ventricular walls of the murine brain regulate glucose homeostasis
a M5 tanycytes are located in the floor of the third ventricle (3 V) at the level of the ventromedial arcuate nucleus (Arc) and ME. Tanycytic subpopulations based on previous classifications are indicated. b Enlarged from the box in a, shows the elongated morphology of the M5 tanycytes projecting to the ME external zone (exz) near the capillary vessels. c Not all vimentin-positive profiles (magenta) are simultaneously GFP-labeled (filled arrowheads), demonstrating that TRMP5 expression delineates a specific subpopulation of tanycytes (unfilled arrowheads). d-g Fine architecture of the M5 tanycytes. (d, detailed in f) Two individual M5 tanycytes (marked by solid and dotted lines) form branched processes with endfeet in close apposition (arrowheads, yellow overlay) to capillary vessels, visualized by CD-31 staining (red). (e, detailed in g) Other M5 tanycytes terminate at the parenchymal surface away from capillary vessels (arrowheads). h–j M5 tanycytes terminate near permeable microvessels (marked by PV-1 staining (red)), either with endfeet covering the microvessels (h, detailed in i) or seen at a distance using SIM microscopy (j and enlarged box). Scale bars: 50 µm (a‒c, e); 30 µm (d, h); 10 µm (f, g); 5 µm (i, j).
