Fig. 7: Acute M5 tanycyte activation promotes insulin release.
From: Bitter taste cells in the ventricular walls of the murine brain regulate glucose homeostasis
a–c M5-DREADD mice were injected with 2 μg CNO into the third ventricle and perfused 1 h later. M5 tanycytes were detected via an anti-HA tag antibody. Note the colocalization of c-Fos and HA tag signal. Scalebar: 50 µm (overview), 10 µm (inset) (result replicated in n = 5 mice). d Insulin release after M5 tanycyte activation. Mice cheek blood obtained at various time points after third ventricle injection of CNO both in WT and M5-DREADD mice. Serum levels of insulin and leptin were tested via Luminex MAGPIX System (n = 4). Note that insulin release increased significantly in M5-DREADD mice compared with WT, data analyzed with 2-tailed t-test. Asterisks indicate *** p < 0.001. e–g Tanycyte activation via CNO i.p. injection three weeks after AAV-DIO2-Cre virus injected into WT-GFP/DREADD mice. Note the colocalization of GFP and c-Fos signal. Inset: GFP was not detected in the pancreatic islet. Scalebars: 50 µm, (n = 3). h Glucose tolerance test between WT-DREADD and WT mice i.c.v. injected with AAV-DIO2-Cre virus (wild type n = 8, WT-DREADD n = 5). Data were analyzed using 2-tailed t-test. Asterisks indicate * p < 0.05, ** p < 0.01. Error bars represent the standard error of the mean. Source data are provided as a Source Data file.
