Fig. 4: Expression and the effect of two male-killing genes.
From: A male-killing gene encoded by a symbiotic virus of Drosophila
Female (A) and male (B) larval salivary glands of D. melanogaster expressing GFP (female, n = 9; male, n = 10), PVMKp1 (ORF4) (female, n = 12; male, n = 9), and Spaid (female, n = 9; male, n = 9). Staining for acetylated histone H4 lysine 16 (H4K16ac; green) indicates male X chromosomes. Staining for phosphorylated histone H2Av (pH2Av; magenta) indicates DNA damage. DAPI indicates DNA (blue). Arrowheads represent the expected location of male X chromosomes deduced from H4K16ac signals.
