Fig. 6: Prediction of the combined treatment response based on non-malignant subtype compositions.

a Heatmap of the normalized cell abundance with fifteen subtypes estimated via CIBERSORTx and clinical records in our HPC cohort. All 44 samples from RBT, NBT, RAT, and NAT groups were deconvolved for estimation. b Average cell compositions in TME among four groups by dividing fifteen subtypes into two groups named as tumor-promoting subtypes and tumor-suppressive types. Error bars represent standard errors of cell constitutions in corresponding groups. The biological independent sample numbers in the four groups were 15, 15, 7, and 7, respectively. c Cartoon plot illustrating the processes for SVM construction from our HPC cohort and application for prospective trails in HPC. d Measurement for the prediction performance of SVM classifier model. The area under receive operating characteristic curve is 0.86 on the training samples. e Heatmap of the normalized cell abundance with 15 subtypes for additional 12 samples. Tag of correct represented the labels from prediction model and true clinical result were identical, while tag of incorrect represented the labels from the prediction model and true clinical result were different. A summary table was shown to summarize the separate and total correction rates. Source data are provided as a Source Data file. f Drug sensitivity of RO-3306 in malignant tumor cells among RBT, NBT, and NAT groups. t-SNE plots of tumor cells annotated into three groups (left top) and colored by normalized sensitivity scores (left bottom). Histogram plots show the distribution of normalized sensitivity scores for tumor cells in three groups, with dashed vertical lines representing the corresponding median scores. g Drug sensitivity of CAL-101 in malignant tumor cells among RBT, NBT and NAT groups. t-SNE plots of tumor cells annotated into three groups (left top) and colored by normalized sensitivity scores (left bottom). Histogram plots show the distribution of normalized sensitivity scores for tumor cells in three groups, with dashed vertical lines representing the corresponding median scores.