Fig. 5: Effects of rs2238574 genotypes on ARID3A expression in K562 cells. | Nature Communications

Fig. 5: Effects of rs2238574 genotypes on ARID3A expression in K562 cells.

From: A regulatory variant at 19p13.3 is associated with primary biliary cholangitis risk and ARID3A expression

Fig. 5

A Sanger sequencing of CRISPR/Cas9-modified and parental K562 cells. B Relative expression of ARID3A in mutated and parental K562 cells (C/C, n = 9; C/A, n = 7; A/A, n = 15). C ChIP-qPCR results showing different enrichment of PPARG, NR2F1 and NR2C2 at the rs2238574-containing region between C/C and A/A K562 cells (n  =  3, biologically independent samples). D Flow cytometric measurement of surface antigen CD117 and CD33 in C/C and A/A K562 cells. Data are representative of three independent experiments (Mean ± SD for quadruplicates). P values are calculated using unpaired two-tailed Student’s t test and indicated significant if *p < 0.05, **p < 0.01, or ***p < 0.001. MFI mean fluorescence intensity.

Back to article page