Fig. 4: Display of grayscale images acquired via fluorescence slide microscopy.

Screenshot of the Slim user interface displaying multiple grayscale images of an immunostained colon adenocarcinoma specimen from the Human Tumor Atlas Network (HTAN) that were acquired via cyclic immunofluorescence imaging, where the user manually selected individual image channels for display and adjusted the display settings for each channel (left). The viewer constructed value of interest and palette color lookup tables from the user-provided display settings to contrast enhance and colorize selected grayscale images, respectively, and additively blended the resulting pseudocolor images (right).