Fig. 2: Enhanced survival of CRs in the juvenile and adult hippocampus. | Nature Communications

Fig. 2: Enhanced survival of CRs in the juvenile and adult hippocampus.

From: Aberrant survival of hippocampal Cajal-Retzius cells leads to memory deficits, gamma rhythmopathies and susceptibility to seizures in adult mice

Fig. 2

a Coronal sections of juvenile (P24) control and BaxCKO (samples carry TaunlsLacZ reporter) hippocampus immunostained for βgal (top panels). Quantification of βgal+ cells in the different hippocampal regions of control and mutant animals (bottom panel) showing increased number of CRs in the mutant hippocampal fissure (HF) (84.34 ± 6.06 cells/mm in controls versus 108.06 ± 8.42 cells/mm in mutants; p = 0.0018) but not vDG (58.94 ± 9.5 cells/mm in control versus 62.75 ± 11.85 in mutants; p = 0.7709) (Two-way ANOVA, Sidak’s multiple comparison test; n = 5 controls and n = 5 mutants). Scale bar: 500 μm. b Coronal sections of adult control and BaxCKO (samples carry TaunlsLacZ reporter) hippocampus immunostained for βgal (top panels). Quantification of βgal+ cells in the different hippocampal regions of control and mutant animals (bottom panel) showing increased number of CRs in mutant HF (54.32 ± 2.41 cells/mm in controls versus 70.85 ± 9.75 cells/mm in mutants) but not in vDG (38.61 ± 10.73 cells/mm in controls versus 39.29 ± 8.52 cells/mm in mutants, p = 0.0348 and 0.9922, respectively, two-way ANOVA, Sidak’s multiple comparison test; n = 4 controls and n = 4 mutants). The dashed line represents the HF including SLM of CA1/CA2/CA3 and OML of the dDG and the dotted line the separation between dDG and vDG. Scale bar: 500 μm. c High magnifications in the CA1 region (squares in b) stained for βgal. Scale bar: 100 μm. d High magnifications in the CA1 region of adult control and BaxCKO upon P73 staining and quantification (bottom panel) indicating an increase in P73+ cells in HF of mutant animals ((p = 0.0009 for HF, p = 0.9984 for vDG, two-way ANOVA, Sidak’s multiple comparison test; n = 3 controls and n = 3 mutants). Scale bar: 100 μm. e High magnification of coronal sections of adult control and BaxCKO (all the samples are carrying the R26Tomato reporter) hippocampus immunostained for Tomato (TOM), RELN and GABA. All TOM+ cells are RELN+ and GABA− (p < 0.0001 for controls; p < 0.0001 for mutants) indicating they are CRs and confirming the increased number in HF, but not vDG in mutant animals (p = 0.0305 for HF, p = 0.2615 for vDG, two-way ANOVA, Sidak’s multiple comparison test; n = 4 controls and n = 4 mutants). Scale bar: 100 μm. All data are presented as mean ± SEM. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).

Back to article page