Fig. 2: GluN1 or GluN2A M4 helices have distinct effects on the pattern of NMDAR gating.
From: Two gates mediate NMDA receptor activity and are under subunit-specific regulation
a Single channel traces of on-cell patches at −100 mV for wild-type (upper trace) or constructs containing alanine (A) substituted at the conserved glycine (G) either in GluN1a(G815A) (middle trace) or GluN2A(G819A) (lower trace). (C)losed and (O)pen states are indicated. Gray bars indicate ‘clusters’, which were defined based on Tcrit. b–d Bar graphs (mean ± SEM with circles indicating individual values) of single channel equilibrium open probability (eq. Popen) (b), cluster Popen (c), and cluster probability (Pcluster = mean cluster duration/(mean cluster duration + mean intercluster duration) (d). e–i Ratio of mean duration (mean ± SEM) between G-A constructs and wild-type for the two slowest closed components, C4 (e) and C5 (f) and for the three fastest components, C1 (g), C2 (h), and C3 (i) (Supplementary Fig. 2). The dashed line is 1, which represents no effect relative to wild-type.**p < 0.01, ***p < 0.001, one-way ANOVA with post-hoc Tukey’s test (b–d) or two-tailed Student’s t test, unpaired (e–i). b ANOVA (p = 8.2E-27): wt vs N1 G-A, p = 5.9E-15; wt vs N2A G-A, p = 5.8E-15; N1 G-A vs N2A G-A, p = 0.99. c ANOVA (p = 2.9E-29): wt vs N1 G-A, p = 4E-15; wt vs N2A G-A, p = 4E-15; N1 G-A vs N2A G-A, p = 0.0004. d ANOVA (p = 1.8E-22): wt vs N1 G-A, p = 0.97; wt vs N2A G-A, p = 1.6E-17; N1 G-A vs N2A G-A, p = 8.6E-14. e–i Two-tailed Student’s t test, unpaired: p = 0.028 (e), 0.00013 (f), 0.0046 (g), 0.47 (h), and 0.24 (i). See Supplementary Tables 2 and 3 for ns and additional parameters.
