Fig. 4: Prying open the M3 gate.
From: Two gates mediate NMDA receptor activity and are under subunit-specific regulation
a On-cell recordings of constructs containing a tyrosine (Y) substituted at A650 in GluN2A, either alone or with G-to-As at the conserved glycines. Records displayed and analyzed as in Fig. 2. b–d Bar graphs (mean ± SEM) for single channel equilibrium Popen (b), cluster Popen (c), and cluster probability (Pcluster) (d). *p < 0.05, ***p < 0.001, one-way ANOVA with post-hoc Tukey’s test. b ANOVA (p = 2.5E-11): A-Y vs N1 G-A, p = 1.3E-11; A-Y vs N2A G-A, p = 1.1E-05; N1 G-A vs N2A G-A, p = 3.2e-05. c ANOVA (p = 2.1E-11): A-Y vs N1 G-A, p = 7E-11; A-Y vs N2A G-A, p = 0.45; N1 G-A vs N2A G-A, p = 10E-10. d ANOVA (p = 5.4E-07): A-Y vs N1 G-A, p = 0.024; A-Y vs N2A G-A, p = 3.7E-07; N1 G-A vs N2A G-A, p = 10E-05. See Supplementary Tables 5 and 6 for ns and additional parameters.
