Fig. 2: OsEPFL6, OsEPFL7, OsEPFL8, and OsEPFL9 redundantly control rice spikelet number per panicle by activating the MAPK cascade.
From: Optimization of rice panicle architecture by specifically suppressing ligand–receptor pairs
a–l Rice panicles from FAZ1 (a) and the osepfl6;7 (b), osepfl6;8 (c), osepfl6;9 (d), osepfl7;8 (e), osepfl7;9 (f), osepfl8;9 (g), osepfl5;6 (h), osepfl5;8 (i), oser1 (j), oser2 (k), and oser1;2 (l) mutants. Scale bar = 5 cm. m, n Comparison of average spikelet number per panicle (m), and setting percentage (n) between FAZ1, osepfl6, osepfl7, osepfl8, osepfl9, osepfl5, osepfl6;7, osepfl6;8, osepfl6;9, osepfl7;8, osepfl7;9, osepfl8;9, osepfl5;6, osepfl5;8, and oser1 mutants. Values are given as the mean ± SD (n = 10 plants). Different letters indicate statistical significance groups at p < 0.05 (one-way ANOVA with post-hoc Tukey’s multiple comparison test). o–r Comparison of OsMPK6 phosphorylation levels in FAZ1, osepfl6;7, osepfl6;8, and oser1 (o); FAZ1, osepfl6;9, osepfl7;9, and oser1 (p); FAZ1, osepfl7;8, osepfl8;9, and oser1 (q); and FAZ1, osepfl5;6, osepfl5;8, and oser1 (r). Proteins extracted from young panicles were analyzed with immunoblot using anti-Phos-OsMPK6 and anti-OsMPK6 antibodies. Anti-Actin antibody was used as the loading control. The graphs show the qualification of the relative levels of the phosphorylated OsMPK6. Values are given as the mean ± SD (n = 3 biological replicates). Different letters indicate statistical significance groups at p < 0.05 (one-way ANOVA with post-hoc Tukey’s multiple comparison test). The source data underlying the statistical analysis in m–r and uncropped images in o–r are provided in the Source Data file.
