Fig. 3: Crosstalk analysis among far-red dyes. | Nature Communications

Fig. 3: Crosstalk analysis among far-red dyes.

From: Single-molecule localization microscopy reveals the ultrastructural constitution of distal appendages in expanded mammalian centrioles

Fig. 3

a Two-color Ex-dSTORM images of C2CD3 labeled with CF568 and Ac-Tub stained with different far-red dyes, displaying a crosstalk effect in the red detection channel. Yellow boxes mark the signals of C2CD3, while blue lines enclose the crosstalk signals from Ac-Tub staining with AF647, ATTO647N, or Dy654. b Quantitative analysis of crosstalk ratio among these far-red dyes with two analytical methods based on single-molecule events or localizations. Both results indicate the minimum crosstalk using Dy654 (mean \(\pm \,\)SD, n = 4 cells for each far-red dye). Scale bar, 500 nm (a). Source data are provided as a Source Data file.

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