Fig. 5: Sporulation-related genes of strain YIM 93972.

a Typical gene expression profiles in varied differentiating conditions at the mRNA and protein levels. Abbreviations: AH, aerial hyphae; SH, substrate hyphae; W, wild type; T, transitional mutant; B, bald mutant. b Gene organization of the peptide ABC transporter in strain YIM 93972. Arrows indicate the size and directions of ORFs. c Expression levels of the peptide ABC transporter encoding genes at the mRNA and protein level. In transcriptomic data, gene expression at the mRNA level between two groups was calculated by TPM value and shown as means±MSD from W-AH/W-SH (n = 3), T-SH/W-SH (n = 6), and B-SH/W-SH (n = 9). In proteomic data, gene expression at the protein level between two groups was calculated by intensity and shown as means ± MSD from W-AH/W-SH (n = 2), T-SH/W-SH (n = 3), and B-SH/W-SH (n = 4). Statistical differences between two groups were calculated using two-tailed unpaired t-tests (inter-group) at the mRNA level and Significance A⁷⁰ (intra-group) at the protein level, respectively. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001. The fold change and p-Value were shown in Supplementary Data 4 for Fig. 5c. d The morphological mutants are resistant to the toxic peptide bialaphos (n = 3). The strains listed above were grown for 14 days on ISP 4 agar plate containing 0 (left plate) and 0.8 (right plate) μg/mL bialaphos.