Fig. 6: Role of peptide permeases in morphological differentiation: complementation of a bldKA-KE deletion mutant of Streptomyces coelicolor with the homologous operon of YIM 93972.
From: Cellular differentiation into hyphae and spores in halophilic archaea
a Comparison of the bldK operon of S. coelicolor M145 with ORF_2669-ORF_2673 of YIM 93972; arrows indicate the size and directions of genes; percentage of amino acid sequence identity is indicated for each gene. b Comparison of mycelia phenotypes among M145/pIB139, ΔbldKA-KE/pIB139, ΔbldKA-KE/pIB139-bldKA-KE, and ΔbldKA-KE/pIB139-ORF_2669-ORF_2673 using scanning EM (bar, 5 μm). Deletion of bldKA-KE in M145 abrogated the development of aerial mycelia and spores; this phenotype was restored to near wild-type levels by complementation with bldKA-KE or ORF_2669-ORF_2673 (n = 3).
