Fig. 4: Factors controlling the network pattern formation revealed by real-time CLSM imaging. | Nature Communications

Fig. 4: Factors controlling the network pattern formation revealed by real-time CLSM imaging.

From: Four distinct network patterns of supramolecular/polymer composite hydrogels controlled by formation kinetics and interfiber interactions

Fig. 4

Time-lapse images of a BPmoc-F3/Alx488-Agarose, c Phos-MecycC5/Alx488-Agarose, e GalNAc-cycC6/Alx488-Agarose, and g DBS-COOH/Alx488-Agarose. Magenta: supramolecular network, green: agarose network. b, d, f, h The quantitative analysis of the network formation. Magenta: the number of supramolecular nanofibers counted by the particle analysis, green: the number of the aggregated agarose domain. i Proposed mechanism of the network pattern formation. Conditions: [BPmoc-F3] = 0.1 wt% (1.6 mM), [Phos-MecycC5] = 0.4 wt% (6.5 mM), [GalNAc-cycC6] = 0.3 wt% (4.6 mM), [Alx488-Agarose] = 0.5 wt%, [TMR-Gua] = 14 µM, [Alx546-cycC6] = 4.0 µM, [DBS-COOH] = 0.2 wt% (4.49 mM), [glucono-δ-lactone] = 44.9 mM (for g, h), solvent: 100 mM MES pH 7.0 (for a–f) or water (for g, h), rt.

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