Fig. 5: Properties of 304 STATIC genes of mouse brain tissue.

a Heatmap of coefficient of variation (CV) of the 304 stable across time in cell-types (STATIC) genes that were identified from 25 cell types in the scRNA-seq data of a mouse brain study (GSE129788³⁶). The CVs were evaluated based on pseudo-bulk intensities in brain tissues from either n = 8 young or n = 8 old mice. b Uniform Manifold Approximation and Projection (UMAP) using only the 304 STATIC genes as input features (sUMAP) on the same scRNA-seq data. Cells are labeled as in the original study. c Percentage of top STATIC genes that overlap with cell-type marker genes identified in the original study. Up to 25 top STATIC genes from each cell type are compared with the corresponding marker genes of the same cell type. d Venn diagram showing the overlap between the 234 STATIC genes identified from 15 out of the 25 cell types and biomarkers distinguishing young versus old mice that were identified in the original study from the same 15 cell types. Adobe Illustrator (version 27.1.1; https://www.adobe.com/products/illustrator.html) was used to arrange panels and edit text. Source data are provided as a Source Data file.