Fig. 5: Activation of GABAA receptors in awake mice, trigger Cl− efflux from astrocytes.
From: Astrocytic chloride is brain state dependent and modulates inhibitory neurotransmission in mice
a Astrocytic [Cl−]i recordings using mClY or MQAE in response to injections of GABA or muscimol. b, f Representative images of the mouse cortex expressing mClY in astrocytes or loaded with MQAE and co-labelling with SR-101. c, g Astrocytic [Cl−]i traces during muscimol injection, shading indicates ±SD. N = 3 mice. d, h Decrease of astrocytic [Cl−]i in response to muscimol or GABA injections. Data represent mean ± SD. N = 4 mClY/Muscimol, 3 mice MQAE/GABA, 10 mice MQAE/Muscimol. e, i Duration of astrocytic [Cl−]i transient upon GABA or muscimol injections. Data represent mean ± SD. N = 4 mClY/Muscimol, 3 mice MQAE/GABA, 10 mice MQAE/Muscimol. j Astrocytic [Cl−]i trace during aCSF injection, mean ± SD. N = 1 mouse, 21 cells. k Change in astrocytic [Cl−]i in response to aCSF injection. N = 1 mouse, 21 cells. l Duration of astrocytic [Cl−]i change upon aCSF injection. N = 1 mouse, 21 cells. [Cl−] = mClY − ΔF/F (%). Data represent mean ± SD. Source data are provided as a Source Data file.
