Table 2 Quality control steps
From: Single-nucleus RNA-sequencing of autosomal dominant Alzheimer disease and risk variant carriers
Performed on | Individual samples | Cell types | ||||||
|---|---|---|---|---|---|---|---|---|
filter applied | Cell ranger | Barcode-rank Distribution | Distrib UMI | Distrib genes | % mit. | >10 cells* | Double finder | Garnett |
Total #nuclei | 1,102,459 | 566,486 | 423,937 | 414,303 | 380,625 | 380,625 | 346,264 | 294,114 |
Mean #genes | 3154 | 2418 | 2242 | 2228 | 2234 | 2234 | 2221 | 2182 |
Mean #UMI | 10,279 | 5646 | 4969 | 4899 | 4897 | 4897 | 4888 | 4725 |
Avg %mitochondria | 2.35 | 2.34 | 1.98 | 1.94 | 1.22 | 1.22 | 1.21 | 1.21 |
