Fig. 3: PE2 variants that incorporate improved nCas9 variants induce a reduced frequency of unwanted indels.

a Plasmids encoding PE (nCas9 variants) and individual pegRNAs targeting 12 endogenous loci were transfected into HEK293T cells. b, c The frequencies of intended edits and unwanted indels at each site are presented in the graphs. Average frequencies of intended edits (b) and unwanted indels (c). d The percentage of unwanted indels among all edited sequences (unwanted indels + intended edits) for the 12 target sites. The red arrows indicate the three variants that reduce the frequency of unwanted indels the most, relative to all edited sequences. Mean ± SEM (a) were determined three independent experiments. Mean ± SEM (b–d) of all individual values of sets of n = 3 independent replicates are shown. Data of minimum-to-maximum values are presented. For the boxes (b–d), the top, middle, and bottom lines represent the 25th, 50th, and 75th percentiles, respectively. The whiskers indicate min to max values. Source data are provided as a Source Data file.