Table 2 Selection of antigens reactive in most Chagas-positive individual serum samples
Cluster | Best antigenic region in cluster | ||||||
|---|---|---|---|---|---|---|---|
# Regions | Sero (%) | Locus identifier | Start—End | Description | Sero (%) | Rep | Best 16mer |
169 | 100 | TCSYLVIO_004530 | 9–244 | Trans-sialidase, putative | 99 | No | GTNSDPDSFSSTNVSG |
3 | 92 | TcCLB.509007.70 | 1249–1308 | Protein phosphatase 2C | 90 | No | AVPNFAAATADKPVGT |
48 | 87 | TcCLB.506289.240 | 161–312 | Hypothetical protein | 55 | No | EGKDERKSGEATAPQV |
8 | 87 | TcCLB.507297.30 | 1–96 | Metacaspase | 86 | No | ERPPRVDVEEFFQQAE |
10 | 83 | TcCLB.506597.33 | 145–193 | Hypothetical protein | 80 | No | LRQIDASPPEPFTAAP |
3 | 80 | TCSYLVIO_004283 | 221–276 | 60S ribosomal protein L4 | 80 | No | KEAMAFLKAIGAVDDV |
2 | 79 | TCSYLVIO_009331 | 481–536 | SMC protein 4 | 79 | No | LRKIDAATERNGNLVA |
1 | 77 | TcCLB.506941.194 | 1025–1080 | Hypothetical protein, conserved | 77 | No | FRKIDAAVPVPNTSYA |
1 | 76 | TcCLB.507809.119 | 81–140 | Autophagy protein Apg6 | 76 | No | VLRKIEEEFQQLEEQK |
2 | 76 | TCSYLVIO_009815 | 281–336 | Hypothetical protein | 76 | No | FRQIDTEHNDRITAEQ |
2 | 75 | TCSYLVIO_001410 | 229–284 | NADH-cytochrome b5 reductase | 75 | No | PPFMEAISGDKDFKTS |
2 | 73 | TCSYLVIO_003288 | 97–152 | Reticulon domain protein | 73 | No | LTSDDIHEAVNRLVDC |
3 | 72 | TcCLB.506441.20 | 641–888 | Cytoskeleton-associated protein | 70 | Yes | REGRERGYPEEKEDSR |
1 | 72 | TcCLB.506925.460 | 41–92 | Kinetoplastid kinetochore protein 16 | 72 | No | LRMIDELAAGVEMWKQ |
3 | 72 | TCSYLVIO_003590 | 89–140 | Microtubule-associated protein Gb4 | 72 | No | LREIDDVENHASQSRA |
23 | 72 | TcCLB.510157.10 | 281–332 | MASP subgroup S022 | 45 | No | AASANKYDTVPQSAGS |
3 | 70 | TcCLB.509139.20 | 37–88 | Hypothetical protein, conserved | 70 | No | SGGAPPTRGGFGAGTS |
2 | 70 | TcCLB.509791.120 | 65–172 | Kinetoplast-associated protein | 70 | No | YDVSKPLDVEKEISKA |
