Fig. 1: Estimating the promoter activities of random sequences in YPD.
From: Chance promoter activities illuminate the origins of eukaryotic intergenic transcriptions
a Experimental procedure. b, c DNA (b) and cDNA (c) counts of each barcode in two experimental replicates. For clarity, 1000 randomly sampled barcodes are shown. Pearson’s correlation (r) and its associated P value based on all barcodes are presented. d Estimated barcode expression levels in two replicates. Expression level is measured by the cDNA count divided by the DNA count of the barcode. The same 1000 barcodes as in (b) and (c) are shown. Pearson’s r and associated P value based on all barcodes are presented. The inset shows the expression levels of 1% of genotypes with the highest cDNA counts and associated statistics. e Expression levels measured by bulk sequencing is strongly correlated with those measured by RT-qPCR in five reconstructed genotypes, a randomly picked negative control, and a randomly picked positive control. Mean expressions and standard errors are shown by dots and error bars, respectively. Pearson’s correlation (r) and the associated P value between the two measurements are presented. All P values are from two-tailed tests. Source data are provided as a Source Data file.
