Fig. 4: Bi-directional clamping of voltage-dependent fluorescence in C. elegans neurons.
From: All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals
A OVC in cholinergic or GABAergic neurons. B Confocal fluorescence z-projections of BiPOLES (mCerulean) and QuasAr2 expression. Arrowheads: Neuronal cell bodies. Scale bars: 50 µm. Representative image (from n = 11 (cholinergic) or 16 (GABAergic neurons)). C OVC protocol (holding values 0, −5, 5% ΔF/F0) in cholinergic neurons, single recording. D, E Mean (±S.E.M.) traces of the OVC in cholinergic (D; n = 14), or GABAergic (E, n = 7) neurons, respectively, holding values: 0, −5, 5% ΔF/F0. F, G Transition times for 5 and 10% ΔF/F0 steps (F: cholinergic, G: GABAergic neurons, n—numbers as in D, E); box plots (median, 25th–75th quartiles), open dot: mean, whiskers: 1.5× IQR. n—numbers refer to independent biological replicates (animals) (D–G). Source data are provided as a Source data file.
