Fig. 3: Sex differential expression of Piezo2 in subtypes of DRG neurons.
From: Piezo2 regulates colonic mechanical sensitivity in a sex specific manner in mice
a The percentage of Piezo2-expressing dorsal root ganglia (DRG) neurons distributes to each category defined by diameters of cell-sizes. Data are from DRG of 3 biologically independent male mice (162 neurons) and DRG of 3 biologically independent female mice (208 neurons). b The numbers of Piezo2-expressing DRG neurons are categorized into different groups according to the size of the neurons by area (µm2). Data are from male (318 neurons measured) and female (503 neurons measured) mice. c, d Double immunostaining of Isolectin-IB4 and Piezo2 in male and female mice. e Summarized data shows IB4 and Piezo2 co-expression in 105 µm2 area of DRG sections of n = 3 biologically independent male and n = 3 biologically independent female mice. Data are presented as mean values + /- SEM. Two-tailed unpaired t test (p = 0.0079, t = 4.92, F = 1.041). Scale bar: 50 μm. f, g Double immunostaining of calcitonin gene-related peptide (CGRP) and Piezo2 in male and female mice. h Summarized data shows CGRP and Piezo2 co-expression in 105 µm2 area of DRG sections of n = 4 biologically independent male and n = 3 biologically independent female mice. Data are presented as mean values + /- SEM. Two-tailed unpaired t test (p = 0.84, t = 0.2126, F = 1.632). Scale bar: 50 μm.
