Fig. 1: Whole brain 3D imaging of viral infection.
From: Type I interferon shapes brain distribution and tropism of tick-borne flavivirus
a A workflow for viral infection and whole brain imaging. Illustration was created using BioRender.com. b Mouse brain appearance in aqueous and in optical clearing solvent, BABB. c Volumetric 3D-render of the brain of mock and LGTV infected brains immunolabeled with anti-NS5 antibody (virus marker; red glow). The signal intensity was normalized within an individual brain and adjusted to identical minimum and maximum. For each image pair, the top and lateral views of the same specimen are shown. Five mice per group were analyzed. Representative images are shown here, and the remaining brains (n = 4) are shown in Supplementary Fig. 1b. Scale bar = 2000 µm. d OPT-MRI fusion image created using viral OPT signal (red) with own MR scan of WT and Ifnar–/– brains. e 3D mapping of viral OPT signal with the OCUM template.
