Fig. 2: Observed enthalpy in different buffer environments compared to heat of ionization shows recruitment of protons upon MPI binding to polyP.
From: Smart thrombosis inhibitors without bleeding side effects via charge tunable ligand design
A Evaluation of free unbound MPI charge content. Potentiometric titrations were used to evaluate the charge content of the MPI library at 25 °C and 160 mM NaCl. A sample titration of 0.15 M NaOH into a solution of acidified MPI 3 while measuring the change in potential is shown. B The speciation plot calculated from titration of MPI 3 with NaOH. C ΔHobserved obtained from binding MPI 3 and polyP (P75) using ITC200 in four buffers plotted against each buffers’ ΔHionization, at pH 7.4, 150 mM NaCl and 25 °C.
